Investigations of Light Intensities, Nutrient, and Carbon Sources Towards Microalgae Oil Production via Soxhlet Extraction Techniques

Abstract

This study was carried out to study the optimized condition for microalgae cultivation in terms of light intensity, nutrient supply, and a carbon source to optimize the microalgae growth to produce microalgae with high biomass productivity and have high lipid content. Microalgae cultivation was carried out with microalgae culture were cultivated under high light intensities and no light condition. The effect of light intensity, NPK fertilizer presence, and glucose presence on microalgae’s biomass production will be observed simultaneously. At the end of cultivation, MX2 obtained the highest biomass, and the biomass was being extracted, which produced 97.186 g of biomass. The oil extraction yield is 9.66%. GC-MS analysis showed the presence of UFA and PUFA in the oil. Thus, future research is needed to improve the technique to increase the microalgae biomass and lipid in the future to become the potential feedstock for the production of biodiesel.