Influence of parity and live weight on the concentration of Pregnancy-Specific Protein B (PSPB) in Kedah Kelantan (KK) cattle

Abstract

The pregnancy-specific protein B (PSPB) is the protein of binucleated cells in the ruminant's placenta. It contains several antigenic variants, which contributed to the ability to detect pregnancy from day 20 of pregnancy until parturition. In this research, the main objective is to determine the concentration of PSPB in serum and urine and their relation to Kedah-Kelantan (KK) cattle's parity and live weight. Ten cattle (n=10) were selected for this study. The blood serum (2 ml) and urine samples were collected at days 0, 24, 42, and 164 post artificial insemination (AI). All the samples were centrifuged at 3000 rpm for 15 minutes at 4°C. The serum and urine were analyzed with competitive ELISA test kit and read at 450 nm wavelength speed. The standard OD value provided by the kit created the equation to calculate the concentration of PSPB in the serum and blood. Then, the correlation and multiple linear regression of parity and live weight were analyzed with a significant level of P < 0.01. Through the finding, the live weight and parity were positive correlate with PSPB in serum. The relationship between live weight and PSPB concentration in urine showed a negative correlation but there no correlation between parity and PSPB concentration in urine. The regression analysis result was 35% and 36% for PSPB concentration in serum and urine, respectively. This study suggests that cattle parity and live weight did not significantly affect the PSPB concentration.