Optimization and Kinetic Analysis on the Production of Hyaluronic Acid by Streptococcus Zooepidemicus in A Batch System

Abstract

In this study, the optimization and kinetic analysis on the effect of process parameters for the production of hyaluronic acid production by S. zooepidemicus were performed in batch system. The fermentation condition such as inoculum size (2 to 20 %), pH (4 to 10), temperature (30 to 45) and agitation (50 to 500) were optimized using "one-factor-at-a-time"method. It was found that the inoculum size of 10 % had the greatest effect on the fermentation process which gave the highest value of specific growth rate, cell biomass and HA production of 0.72 h-1, 1.96 gl-1 and 0.82 gl-1, respectively. Furthermore, the other process parameters such as pH, temperature and agitation speed were also found to improve the cell biomass, HA production and kinetic analysis. The highest cell biomass (0.17 gcell gglucose-1) and HA yield (0.096 gHA g glucose-1) were obtained at pH 7.0, 37 °C and agitation speed 300 rpm for 12 h. The overall kinetics analysis of maximum specific growth rate, volumetric cell biomass productivity and volumetric HA productivity obtained at optimum parameters were, 0.81 h-1, 0.183 gcell l-1 h-1 and 0.111 gHA l-1 h-1, respectively.