Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/5012
DC Field | Value | Language |
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dc.contributor.author | Noorul Izzati Hanafi | en_US |
dc.contributor.author | Maizan Mohamed | en_US |
dc.contributor.author | Kuttulebbai Naina Mohamed Salam Sirajudeen | en_US |
dc.contributor.author | Noor Hafizoh Saidan | en_US |
dc.contributor.author | Gan Siew Hua | en_US |
dc.contributor.author | Khomaizon Abdul Kadir Pahirulzaman | en_US |
dc.contributor.author | Pasupuleti Visweswara Rao | en_US |
dc.date.accessioned | 2023-11-15T04:29:14Z | - |
dc.date.available | 2023-11-15T04:29:14Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/5012 | - |
dc.description | Web of Science | en_US |
dc.description.abstract | Objective(s): This study aimed to investigate the protective effects of fenugreek on CoCl2-induced hypoxia in neonatal rat cardiomyocytes. Materials and Methods: Primary cardiomyocytes were isolated from Sprague Dawley rats aged 0–2 days and incubated with various concentrations of fenugreek (10-320 µg/ml) and CoCl2-induced hypoxia for different durations (24, 48, and 72 hr). Cell viability, calcium signaling, beating rate, and gene expression were evaluated. Results: Fenugreek treatments did not cause any toxicity in cardiomyocytes. At a concentration of 160 µg/ml for 24 hr, fenugreek protected the heart against CoCl2-induced hypoxia, as evidenced by reduced expression of caspases (-3, -6, -8, and -9) and other functional genes markers, such as HIF-1α, Bcl-2, IP3R, ERK5, and GLP-1r. Calcium signaling and beating rate were also improved in fenugreek-treated cardiomyocytes. In contrast, CoCl2 treatment resulted in up-regulation of the hypoxia gene HIF-1α and apoptotic caspases gene (-3, -9, -8, -12), and down-regulation of Bcl-2 activity. Conclusion: Fenugreek treatment at a concentration of 160 µg/ml was not toxic to neonatal rat cardiomyocytes and protected against CoCl2-induced hypoxia. Furthermore, fenugreek improved calcium signaling and beating rate and altered gene expression. Fenugreek may be a potential therapeutic agent for promoting cardioprotection against hypoxia-induced injuries. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Mashhad University of Medical Sciences | en_US |
dc.relation.ispartof | Iranian Journal of Basic Medical Sciences | en_US |
dc.subject | Cardiomyocytes | en_US |
dc.subject | Hypoxia | en_US |
dc.subject | Ischemia | en_US |
dc.subject | Therapeutics | en_US |
dc.title | Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes | en_US |
dc.type | National | en_US |
dc.identifier.doi | 10.22038/IJBMS.2023.71521.15547 | - |
dc.description.page | 1360-1369 | en_US |
dc.volume | 26(11) | en_US |
dc.description.type | Article | en_US |
dc.description.impactfactor | 2.2 | en_US |
dc.description.quartile | Q2 | en_US |
dc.contributor.correspondingauthor | khomaizon@umk.edu.my | en_US |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.openairetype | National | - |
crisitem.author.dept | Universiti Malaysia Kelantan | - |
crisitem.author.dept | University Malaysia Kelantan, Malaysia | - |
Appears in Collections: | Faculty of Agro Based Industry - Journal (Scopus/WOS) |
Files in This Item:
File | Description | Size | Format | |
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Promoting cardioprotection with fenugreek-Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes.pdf | 454.58 kB | Adobe PDF | View/Open |
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