Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/4712
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dc.contributor.authorSaeed S.I.en_US
dc.contributor.authorVivian L.en_US
dc.contributor.authorZalati C.W.S.C.W.en_US
dc.contributor.authorSani N.I.M.en_US
dc.contributor.authorAklilu, E.en_US
dc.contributor.authorMohamed, M.en_US
dc.contributor.authorNoor A.M.en_US
dc.contributor.authorMuthoosamy K.en_US
dc.contributor.authorKamaruzzaman, N.en_US
dc.date.accessioned2023-08-01T07:31:47Z-
dc.date.available2023-08-01T07:31:47Z-
dc.date.issued2023-
dc.identifier.issn17466148-
dc.identifier.urihttp://hdl.handle.net/123456789/4712-
dc.descriptionWeb of Science / Scopusen_US
dc.description.abstractBackground: S. aureus is one of the causative agents of bovine mastitis. The treatment using conventional antimicrobials has been hampered due to the development of antimicrobial resistance and the ability of the bacteria to form biofilms and localize inside the host cells. Objectives: Here, the efficacy of graphene oxide (GO), a carbon-based nanomaterial, was tested against the biofilms and intracellular S. aureus invitro. Following that, the mechanism for the intracellular antimicrobial activities and GO toxicities was elucidated. Methods: GO antibiofilm properties were evaluated based on the disruption of biofilm structure, and the intracellular antimicrobial activities were determined by the survival of S. aureus in infected bovine mammary cells following GO exposure. The mechanism for GO intracellular antimicrobial activities was investigated using endocytosis inhibitors. GO toxicity towards the host cells was assessed using a resazurin assay. Results: At 100 ug/mL, GO reduced between 30 and 70% of S. aureus biofilm mass, suggesting GO’s ability to disrupt the biofilm structure. At 200 ug/mL, GO killed almost 80% of intracellular S. aureus, and the antimicrobial activities were inhibited when cells were pre-treated with cytochalasin D, suggesting GO intracellular antimicrobial activities were dependent on the actin-polymerization of the cell membrane. At < 250 ug/mL, GO enhanced the viability of the Mac-T cell, and cells were only affected at higher dosages. Conclusion: The in vitro efficacy of GO against S. aureus in vitro suggested the compound could be further tested in Vivo to zrecognize its potential as one of the components of bovine mastitis therapy.en_US
dc.publisherBioMed Central Ltden_US
dc.relation.ispartofBMC Veterinary Researchen_US
dc.subjectGraphene oxideen_US
dc.subjectIntracellular S. aureusen_US
dc.subjectMastitisen_US
dc.titleAntimicrobial activities of graphene oxide against biofilm and intracellular Staphylococcus aureus isolated from bovine mastitisen_US
dc.typeNationalen_US
dc.identifier.doi10.1186/s12917-022-03560-6-
dc.volume19(1)en_US
dc.description.articleno10en_US
dc.description.typeArticleen_US
dc.description.impactfactor2.6en_US
dc.description.quartileQ1en_US
dc.contributor.correspondingauthornorfadhilah@umk.edu.myen_US
item.openairetypeNational-
item.fulltextNo Fulltext-
item.grantfulltextnone-
crisitem.author.deptFaculty of Veterinary Medicine UMK-
crisitem.author.deptUniversiti Malaysia Kelantan-
crisitem.author.deptUniversiti Malaysia Kelantan-
Appears in Collections:Faculty of Veterinary Medicine - Journal (Scopus/WOS)
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