The Study of Nutritional Factor on The Production of Biosurfactant from Locally Isolated Strain Pseudomonas sp. DSB7

Abstract

This study was aimed to investigate different nutritional factors including carbon source, nitrogen source and hydrocarbon source towards the production of biosurfactant by Pseudomonas sp DSB7. The strain was isolated from soil sediment of Sungai Dungun estuary and 16S rRNA analysis revealed that the isolate was closely related to Pseudomonas aeruginosa. To study the effect of nutritional factor on the production of biosurfactant, the strain was supplemented with different carbon and nitrogen sources and the production of biosurfactant was evaluated by emulsification index. Highest emulsification index was recorded when the strain was grown in minimal salt media (MSM) formulated with sucrose and yeast extract. To study the effect of hydrocarbon as the inducer for biosurfactant production by Pseudomonas sp DSB7, engine oil and kerosene was added to the formulated MSM media. Emulsification index showed that engine oil was a better inducer for biosurfactant production by isolate DSB7.