Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/3644
DC Field | Value | Language |
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dc.contributor.author | Norpi A.S.M. | en_US |
dc.contributor.author | Nordin, M. L. | en_US |
dc.contributor.author | Ahmad N. | en_US |
dc.contributor.author | Katas H. | en_US |
dc.contributor.author | Fuaad A.A.-H.A. | en_US |
dc.contributor.author | Sukri A.. | en_US |
dc.contributor.author | Marasini N. | en_US |
dc.contributor.author | Azmi F. | en_US |
dc.date.accessioned | 2022-12-07T07:00:09Z | - |
dc.date.available | 2022-12-07T07:00:09Z | - |
dc.date.issued | 2022-05 | - |
dc.identifier.issn | 18180876 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/3644 | - |
dc.description | Web of Science / Scopus | en_US |
dc.description.abstract | An effective vaccine against group A streptococcus (GAS) is highly desirable for definitive control of GAS infections. In the present study, two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were developed. The vaccines were primarily composed of encapsulated KLH protein (a source of T helper cell epitopes) and lipidated M-protein derived B cell peptide epitope (lipoJ14) within the amphiphilic structure of nanoparticles. The only difference between them was one of the nanoparticles vaccines received additional surface coating with poly (I:C). The formulated vaccines exhibited nanosized particles within the range of 220–240 nm. Cellular uptake study showed that nanoparticles vaccine without additional poly (I:C) coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly (I:C). Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 cells. Upon immunization in mice, both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical mixture. These antibodies elicited high opsonic activity against the tested GAS strains. Overall, our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly (I:C). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Shenyang Pharmaceutical University | en_US |
dc.relation.ispartof | Asian Journal of Pharmaceutical Sciences | en_US |
dc.subject | Amphiphilic chitosan nanoparticles | en_US |
dc.subject | Group A streptococcus | en_US |
dc.subject | Immunogenicity | en_US |
dc.subject | Lipidation | en_US |
dc.subject | Multi-adjuvanting delivery system | en_US |
dc.subject | Peptide vaccine | en_US |
dc.title | New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus | en_US |
dc.type | Printed | en_US |
dc.identifier.doi | 10.1016/j.ajps.2022.04.002 | - |
dc.description.page | 435 - 446 | en_US |
dc.volume | 17 (3) | en_US |
dc.description.type | Article | en_US |
dc.description.impactfactor | 9.289 | en_US |
dc.description.quartile | Q1 | en_US |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairetype | Printed | - |
item.languageiso639-1 | en | - |
Appears in Collections: | Faculty of Veterinary Medicine - Journal (Scopus/WOS) |
Files in This Item:
File | Description | Size | Format | |
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New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus.pdf | 2.88 MB | Adobe PDF | View/Open |
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