Viability of bovine opu-derived oocytes to honeybee as cryoprotectant

Abstract

This study designs to determine the effectiveness of Honeybee (HB) as cryoprotectants (CP) on viability vitrified-thawed bovine oocytes derived from OPU using Trehalose as control. Cattles were subjected to superstimulation protocol, per session conducted five days where three days both cattle were administrated 100 mg follicle stimulation hormone (FSH) within 24 h once and two days of "resting period" totalling two sessions. The "coasting period" (FSH starvation) between sessions was four days (96 h). Oocytes collection via OPU were performed at fifth day (120 h). The ovarian growth was observed via ultrasonographic before OPU. Prior to vitrifying oocytes with treatment Trehalose (T1) and HB (T2) followed by warming protocol, oocytes subjected to in vitro maturation (IVM). Oocytes viability were evaluated by fluorescein diacetate staining. Results showed ovarian growth for first session was larger size follicles than the second session for both cattle. Total number of oocytes obtained were 60. Oocytes viability treatment T2 was significantly higher (90.9%) than T1 (70.4%). This study concludes that HB as CP in vitrification protocol was able to achieved high oocytes viability with oocytes derived via OPU suggesting Honeybee as an alternative CP for oocytes vitrification.