Effect of Encapsulation-Dehydration Cryopreservation on Histological Analysis of Oncidium Golden Anniversary orchid PLBs

Abstract

Oncidium Golden Anniversary is a hybrid orchid that has been grown as cut flowers, potted plant and is valued for its attractive flower shapes and colours. New hybrid orchids are difficult to maintain and cultivate easily; therefore, cryopreservation technology has been applied to conserve the orchid plant genetic resources. This study aims to evaluate the impact of encapsulation-dehydration as one of the cryopreservation techniques on histological analysis of Oncidium Golden Anniversary orchid PLBs using stock, cryopreserved and non-cryopreserved PLBs. Results from the histological analysis indicated that the degree of plasmolysis due to dehydration and ruptured cells after freezing were the main factors affecting the viability of PLBs.